[dr Radivoje M. Prodanovic]

Hemijski fakultet, Studentski trg 12-16, Beograd
Katedra za biohemiju

Telefon:   011-3336660 (Kabinet), 011-3336663 (Laboratorija)

Telefon (lokalni):   660 (Kabinet), 663 (Laboratorija)

Kabinet:   425

Laboratorija:   434

E-mail:   rprodano@chem.bg.ac.rs

Lični podaci

Rođen 13.11.1972. godine u Šapcu. Oženjen i ima dvoje dece. Govori srpski (maternji) i engleski jezik. Osnovno poznavanje nemačkog i ruskog jezika.

Obrazovanje i usavršavanja

1992-1996: Osnovne studije - Hemijske nauke / Univerzitet u Beogradu - Hemijski fakultet (Beograd)

1996-2001: Magistratura - Hemijske nauke / Univerzitet u Beogradu - Hemijski fakultet (Beograd)

2002-2006: Doktorat - Hemijske nauke / Univerzitet u Beogradu - Hemijski fakultet (Beograd)

Kretanje u službi

1999-2003: Asistent pripravnik / Univerzitet u Beogradu - Hemijski fakultet (Beograd)

2003-2007: Asistent / Univerzitet u Beogradu - Hemijski fakultet (Beograd)

2007-2012: Naučni saradnik / Univerzitet u Beogradu - Hemijski fakultet (Beograd)

2010-2015: Docent / Univerzitet u Beogradu - Hemijski fakultet (Beograd)

2014-2019: Vanredni profesor / Univerzitet u Beogradu - Hemijski fakultet (Beograd)

2019: Redovni profesor / Univerzitet u Beogradu - Hemijski fakultet (Beograd)

Oblasti naučnog interesovanja

Bioinformatika, High-Throughput Screening (FACS). Dirigovana evolucija i Proteinski inženjering. Nevodena enzimologija. Primena enzima u organskim sintezama. Prečišćavanje proteina. Imobilizacija i hemijske modifikacije enzima. Hidrogelovi.

Nagrade i priznanja

1997: Nagrada Srpskog hemijskog društva za izuzetan uspeh tokom studija Biohemije
https://www.shd.org.rs/

2002: Nagrada Vlade Republike Srbije za najboljeg asistenta pripravnika Hemijskog fakulteta

2007: Stipendija Humboldtove Fondacije za projekat "Dirigovana evolucija glukoza oksidaze za primenu u minijaturnim biogorivnim uređajima"School of Engineering and Science, Jacobs University Bremen.
http://www.biotec.rwth-aachen.de/cms/~imne/BIOTEC/?lidx=1

2012: Fulbright-ova stipendija za projekat "Ultrahigh throughput screening system in drop-based microfluidics for directed evolution of glucose oxidase", Harvard University, Boston, USA.
https://weitzlab.seas.harvard.edu/

2019: Stipendija Instituta za napredne studije, Loughborough Univerziteta, Leicester, Velika Britanija
https://www.lboro.ac.uk/research/ias/open-programme/

Ostale aktivnosti

Prodekan za finansije Hemijskog fakulteta od 1. 10. 2015. do 30. 9. 2020.

Član Komisije za master i doktorske studije od 12. 7. 2021.

Član Veća Centra za molekularne nauke o hrani od 12. 5. 2022. do 10. 1. 2024.

Predsednik rukovodilac istraživačkog tima za biotehnologiju i enzimologiju hrane od 12. 5. 2022.

Član Saveta Hemijskog fakulteta od 22. 6. 2023.

Član Veća Centra za molekularne nauke o hrani od 11. 1. 2024.

Član Etičke komisije od 11. 7. 2024.

Angažman u nastavi

Učešće u naučnoistraživačkim projektima

Aktivni projekti:

Završeni projekti:

  • 2011: Alergeni, antitela, enzimi i mali fiziološki značajni molekuli: dizajn, struktura, funkcija i značaj – 172049
    vrsta projekta:   domaći fundamentalni
    projekat finansira:   Ministarstvo za nauku i tehnološki razvoj Republike Srbije (Beograd)
  • 2011: Ispitivanja odnosa struktura-funkcija u ćelijskom zidu biljaka i izmene strukture zida enzimskim inženjeringom – 173017
    vrsta projekta:   domaći fundamentalni
    projekat finansira:   Ministarstvo za nauku i tehnološki razvoj Republike Srbije (Beograd)
  • 2013-2017: Sistemska biokataliza – CMST COST Action CM1303
    vrsta projekta:   međunarodni
    projekat finansira:   Evropska unija (Brisel, Belgija)
  • 2016: Razvoj visoko efikasnih skrining sistema zasnovanih na protočnoj citometriji i mikrofluidici za dirigovanu evoluciju glukoza oksidaze, celobiozo dehidrogenaze i hemicelulaze – 451-03-01038/2015-09/21   (rukovodilac projekta)
    vrsta projekta:   međunarodni
    projekat finansiraju:   Deutscher Akademischer Austauschdienst (Berlin, Nemačka), Ministarstvo prosvete, nauke i tehnološkog razvoja (Beograd)
  • 2017: Razvoj kreme za regeneraciju i protiv starenja kože na bazi puževe sluzi – 391-00-16/2017-16/4 IP   (rukovodilac projekta)
    vrsta projekta:   domaći inovacioni
    projekat finansira:   Ministarstvo prosvete, nauke i tehnološkog razvoja (Beograd)
  • 2020: Kontrolisan dizajn efikasnih enzim@MOF kompozita za primenu u biokatalizi (SYMBIOSIS) – 6066997
    vrsta projekta:   PROMIS
    projekat finansira:   Fond za nauku Republike Srbije (Beograd)
  • 2020: Terapija RNK-viroza malim molekulima. Prenamenovanje iminošećera i hlorokinskih analoga protiv kovida 19 (SMART Repurposing) – 7547552
    vrsta projekta:   COVID-19
    projekat finansira:   Fond za nauku Republike Srbije (Beograd)
  • 2021: Prenamena lekova za prevenciju i lečenje Covid-19 (COVIDTARGET) – 7551100
    vrsta projekta:   COVID-19
    projekat finansira:   Fond za nauku Republike Srbije (Beograd)
  • 2020: Novi pristup lečenju intoksikacije toksinom tetanusa – PoC-5132
    vrsta projekta:   Dokaz koncepta
    projekat finansira:   Fond za inovacionu delatnost Republike Srbije (Beograd)

Brojevi za identifikaciju autora

Izabrane reference

Articles (from SCI list): 75

Cited by other authors: 1731

All citations per article: 23.1

h-index (all citations): 19

View Google Scholar profile

View withdrawn preprint article: Use of microfluidics for directed evolution of enzymes and materials in droplets and microgels

KNJIGE I REVIJALNI RADOVI / BOOKS AND REVIEWS

 

1. Radivoje Prodanović, “Proteinski inženjering”, Izdavač Hemijski fakulteta, 2019. ISBN: 978-86-7220-098-0. Štampa i povez: Službeni glasnik, Beograd.

 

2. N. Milosavic, R. Prodanovic, "Enzyme Stabilization and Imobilization: Methods and Protocols", Methods in Molecular Biology, 679: 155-163 (2010).

 

3. G. Güven, R. Prodanovic, U. Schwaneberg, "Protein Engineering-An Option for Enzymatic Biofuel Cell Design", Electroanalysis, 22: 7-8, 765-775 (2010).

 

4. R.Prodanovic, Tanja Cirkovic Velickovic, "Enzimologija: Laboratorijski priručnik", Hemijski fakultet Univerziteta u Beogradu, (2005) ISBN 86-7220-020-92.

 

IZABRANI RADOVI / SELECTED REFERENCES

 

1. Balaž Ana Marija, Blažić Marija, Popović Nikolina, Prodanović Olivera, Ostafe Raluca, Fischer Rainer, Prodanović Radivoje*, "Expression, purification and characterization of cellobiose dehydrogenase mutants from Phanerochaete chrysosporium in Pichia pastoris KM71H strain", Journal of the Serbian Chemical Society, (2019). https://doi.org/10.2298/JSC190320058B

 

2. Blažić, M., Balaž, A.M., Tadić V., Draganić, B., Ostafe, R., Fischer, R., Prodanović, R.*, "Protein engineering of cellobiose dehydrogenase from Phanerochaete chrysosporium in yeast Saccharomyces cerevisiae InvSc1 for increased activity and stability", Biochemical Engineering Journal, 146: 179-185 (2019).

 

3. Kovačević, G., Ostafe, R., Fischer, R., Prodanović, R.*, "Influence of methionine residue position on oxidative stability of glucose oxidase from Aspergillus niger", Biochemical Engineering Journal 146:143-149 (2019).

 

4. Kovačević, G., Ostafe, R., Balaž, A.M., Fischer, R., Prodanović, R., "Development of GFP-based high-throughput screening system for directed evolution of glucose oxidase", Journal of Bioscience and Bioengineering 127: 30-37 (2019).

 

5. Balaž, A.M., Stevanović, J., Ostafe, R., Blažić, M., Ilić Đurđić, K., Fischer, R., Prodanović, R.*, “Semi-rational design of cellobiose dehydrogenase for increased stability in the presence of peroxide”, Molecular Diversity (2019). https://doi.org/10.1007/s11030-019-09965-0

 

6. Spasojevic, D., Prokopijevic, M., Prodanovic, O. Zelenović, N., Polović, N., Radotić, K., Prodanović, R.*, ”Peroxidase-Sensitive Tyramine Carboxymethyl Xylan Hydrogels for Enzyme Encapsulation”, Macromolecular Research (2019). https://doi.org/10.1007/s13233-019-7111-7

 

7. Blažić, M., Balaž, A.M., Prodanović, O., Popović, N., Ostafe, R., Fischer, R., Prodanović, R.*, "Directed Evolution of Cellobiose Dehydrogenase on the Surface of Yeast Cells Using Resazurin-Based Fluorescent Assay", Applied Sciences, 9:1413 (2019).

 

8. Milošević, J., Janković, B., Prodanović, R., Polović, N., "Comparative stability of ficin and papain in acidic conditions and the presence of ethanol", Amino Acids, 51 (5): 829-838 (2019).

 

9. Menghiu, G., Ostafe, V., Prodanovic, R., Fischer, R., Ostafe, R., „Biochemical characterization of chitinase A from Bacillus licheniformis DSM8785 expressed in Pichia pastoris KM71H“, Protein Expression and Purification 154, pp. 25-32 (2019).

 

10. Prokopijevic M, Prodanovic O, Spasojevic D, Kovacevic G, Polovic N, Radotic K, Prodanović R*, "Tyramine-modified pectins via periodate oxidation for soybean hull peroxidase induced hydrogel formation and immobilization", Applied Microbiology and Biotechnology, 101 (6): 2281-2290 (2017).


11. Spasojević D, Zmejkoski D, Glamočlija J, Nikolić M, Soković M, Milošević V, Jarić I, Stojanović M, Marinković E, Barisani-Asenbauer T, Prodanović R, Jovanović M, Radotić K, "Lignin model compound in alginate hydrogel: a strong antimicrobial agent with high potential in wound treatment", International Journal of Antimicrobial Agents 48 (6): 732-735 (2016).

 

12. Djokic L, Spasic J, Jeremic S, Vasiljevic B, Prodanovic O, Prodanovic R, Nikodinovic-Runic, J. "Immobilization of Escherichia coli cells expressing 4-oxalocrotonate tautomerase for improved biotransformation of β-nitrostyrene", Bioprocess and Biosystems Engineering, 38 (12): 2389-2395 (2015).

 

13. Popovic M., Prodanovic R., Ostafe R., Schillberg S., Fischer R., Gavrovic-Jankulovic M., "Yeast surface display is a novel tool for the rapid immunological characterization of plant-derived food alergens", Immunologic Research, 61 (3): 230-239 (2015).

 

14. Chen Q, Utech S, Chen D, Prodanovic R, Lin JM, Weitz DA. ,"Controlled assembly of heterotypic cells in a core-shell scaffold: Organ in a droplet", Lab on a Chip - Miniaturisation for Chemistry and Biology. 16 (8): 1346-1349 (2016).


15.Prodanovic O, Spasojevic D, Prokopijevic M, Radotic K, Markovic N, Blazic M, Prodanovic R*, "Tyramine modified alginates via periodate oxidation for peroxidase induced hydrogel formation and immobilization", Reactive and Functional Polymers 93:77-83 (2015).

 

16. Utech S, Prodanovic R, Mao AS, Ostafe R, Mooney DJ, Weitz DA., "Microfluidic Generation of Monodisperse, Structurally Homogeneous Alginate Microgels for Cell Encapsulation and 3D Cell Culture", Advanced Healthcare Materials 4 (11): 1628-1633 (2015).

 

17. Tadić Vojin M., Balaž Ana Marija J., Petrić Marija P., Milošević Snežana M., Zelenović Nevena D., Raspor Martin Z., Tadić Jovan M., Prodanović Radivoje M., "Cloning of the gene for a carbohydrate oxidase from Lactuca sativa in the yeasts Saccharomyces cerevisiae and Pichia pastoris", Hemijska Industrija, 69 (6): 689-701 (2015).

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18. R. Ostafe, R. Prodanovic, W.L. Ung, D.A. Weitz, R. Fischer, "A high-throughput cellulase screening system based on droplet microfluidics", Biomicrofluidics, 8: 041102, (2014).

 

19. R. Ostafe, R. Prodanovic, J. Nazor, R. Fischer, "Ultra-High-Throughput Screening Method using Fluorescence Activated Cell Sorting for the Directed Evolution of Glucose Oxidase", Chemistry & Biology, 21: 414-421 (2014).  

 

20. G. Kovacevic, M. Blazic, B. Draganic, R. Ostafe, M. Gavrovic-Jankulovic, R. Fischer, R. Prodanovic*, "Cloning, Heterologous Expression, Purification and Characterization of M12 Mutant of Aspergillus niger Glucose Oxidase in Yeast Pichia pastoris KM71H.", Molecular Biotechnology, 56: 305-311 (2014).

 

21. M. Prokopijevic, O. Prodanovic, D. Spasojevic, Z. Stojanovic, K. Radotic, R. Prodanovic*, "Soybean hull peroxidase immobilization on macroporous glycidyl methacrylates with different surface characteristics", Bioprocess and Biosystem Engineering, 37(5): 799-804 (2014).

 

22. M. Blazic, G. Kovacevic, O. Prodanovic, R. Ostafe, M. Gavrovic Jankulovic, R. Fischer, R. Prodanovic, "Yeast surface display for the expression, purification and characterization of wild-type and B11 mutant glucose oxidases", Protein Purification and Expression, 89: 175-180 (2013).

 

23. R. Ostafe, R. Prodanovic, U. Commandeur, R. Fischer, "Flow Cytometry-Based Ultra-High-Throughput Screening Assay for Cellulase Activity", Analytical Biochemistry, 435: 93-98 (2013).

 

24. O. Prodanovic, M. Prokopijevic, D. Spasojevic, Z. Stojanovic, K. Radotic, Z. Knezevic-Jugovic, R. Prodanovic, "Improved Covalent Immobilization of Horseradish Peroxidase on Macroporous Glycidyl Methacrylate-Based Copolymers", Applied Biochemistry and Biotechnology, 168: 1288-1301 (2012).

 

25. R. Prodanovic*, R. Ostafe, M. Blanusa, U. Schwaneberg, "Vanadium bromoperoxidase-coupled fluorescent assay for flow cytometry sorting of glucose oxidase gene libraries in double emulsions", Analytical and Bioanalytical Chemistry, 404: 1439-1447 (2012).

 

26. D. Despotovic, Lj. Vojcic,R. Prodanovic, R. Martinez, Karl-Heinz Maurer, U. Schwaneberg, "Fluorescent Assay for Directed Evolution of Perhydrolases", Journal of Biomolecular Screening, 17 (6): 796-805 (2012).

 

27. R. Tu, R. Martinez, R. Prodanovic, M. Klein, U. Schwaneberg, "A Flow Cytometry-Based Screening System for Directed Evolution of Protease", Journal of Biomolecular Screening, 16(3): 285-294 (2011).

 

28. R. Prodanovic, R. Ostafe, A. Scacioc, U. Schwaneberg, "Ultrahigh-Throughput Screening System for Directed Glucose Oxidase Evolution in Yeast Cells", Combinatorial Chemistry & High Throughput Screening, 14(1):55-60 (2011).

 

29. W. Lee, Lj. Vojcic, D. Despotovic, R. Prodanovic, Karl-Heinz Maurer, U. Schwaneberg, Martin Zacharias, "Rationalizing perhydrolase activity of aryl-esterase and subtilisin Carlsberg mutants by molecular dynamics simulations of the second tetrahedral intermediate state", Theoretical Chemistry Accounts, 125: 375-386 (2010).

 

30. Z. Zhu, M. Wang, A. Gautam, J. Nazor, C. Momeu, R. Prodanovic, U. Schwaneberg, "Directed evolution of glucose oxidase from Aspergillus niger for ferrocenemethanol-mediated electron transfer", Biotechnology Journal, 2: 241-248 (2007).

 

31. Z. Knežević, N. Milosavić, D. Bezbradica, Ž, Jakovljević, R. Prodanović, "Immobilization of lipase from Candida rugosa on Eupergit® C supports by covalent attachment", Biochemical Engineering Journal, 30: 269-278 (2006).

 

32. R. Prodanović*, N. Milosavić, S. Jovanović, O. Prodanović, T. Ćirković Veličković, Z. Vujčić, R.M. Jankov, "Activity and stability of soluble and immobilized α-glucosidase from baker’s yeast in cosolvent systems", Biocatalysis and Biotransformation, 24: 195-200 (2006).

 

33. R. Prodanović*, N. Milosavić, D. Sladić, M. Zlatović, B. Božić, T. Ćirković Veličković, Z. Vujčić, "Transglucosylation of hydroquinone catalysed by α-glucosidase from baker’s yeast", Journal of the Molecular Catalysis B: Enzymatic, 35: 142-146 (2005).

 

34. R. Prodanović*, N. Milosavić, D. Sladić, T. Ćirković Veličković, Z. Vujčić, "Synthesis of hydroquinone-α-glucoside by α-glucosidase from baker’s yeast", Biotechnology Leters, 27: 551-554 (2005)

 

35. R. Prodanović*, S. Jovanović, Z. Vujčić, "Immobilization of invertase on a new type of macroporous glycidyl methacrylate", Biotechnology Letters, 27: 1171-1174 (2001).