Analytical Chemistry 2   (302B1)

Academic year:

2019/2020.

Attendance requirements:

101A1

ECTS:

8

Study level:

Study program:

Teacher:

Tatjana Ž. Verbić, Ph.D.
associate professor, Faculty of Chemistry, Studentski trg 12-16, Beograd

Assistant:

Olivera S. Marković, Ph.D.
research associate, Institute of Chemistry, Technology and Metallurgy, Njegoševa 12, Beograd

Hours of instruction:

Weekly: two hours of lectures + one hour of exercises + four hours of labwork (2+1+4)

Goals:

Within Analytical Chemistry 2, students will acquire theoretical and practical knowledge in the field of electrochemical and optical methods of analysis. Students will be able to independently perform measurements and determinations within basic electrochemical methods (conductometry, potentiometry, electrolysis and polarography) and optical methods (UV/VIS spectrophotometry, spectrofluorometry and emission and absorption by flame photometry). Within this course students will become familiar with electrochemical biosensors and nanosensors and how they can be applied in on-line and in-vivo measurements. Within group exercises, after they have been introduced to the principles of a method, students present the results of measurements by drawing graphs and doing calculations and later apply the acquired knowledge in processing the results of individual exercises.

Outcome:

Upon completing this course, students will be able to properly choose and apply the most suitable micro- and macro-instrumental methods in the analysis of a complex sample. The theoretical and practical knowledge acquired in this course will enable students to better understand and master the courses in the subsequent years of studies, where the use of instrumental methods of analysis is inevitable.

Teaching methods:

Lectures, theory exercises and experimental exercises (group and individual).

Extracurricular activities:

As a part of their extracurricular activities, students are expected to finish graphs and all exercises (both group and individual) in case they have not finished them during the classes.

Coursebooks:

Main coursebooks:

1. M. Todorović, V. Antonijević i P. Đurđević: Optičke metode, Beograd
2. D. Manojlović, J. Mutić, D. Šegan: Osnove elektroanalitičke hemije, HF, Beograd, 2010.
3. D. A. Skoog, D. M. West, F. J. Holler: Osnove analitičke kemije, Školska knjiga Zagreb, Hrvatska, 1999.

Supplementary coursebooks:

1. G. Bendre: Principles of Chemical Instrumentation, W. B. Saunder Company, Philadelphia, 1987.
2. M. Todorović, V. Antonijević: Zbirka zadataka iz instrumentalne analize, Hemijski fakultet, Beograd, 1998.

Additional material:

http://helix.chem.bg.ac.rs/~tatjanad/Analiticka_hemija_2--302B1/

Lectures:

0 points (2 hours a week)

Syllabus:

1. Optical methods. Properties of electromagnetic radiation. Absorption and emission.
2. Emission methods. The cathode-ray tube. Flame photometry.
3. Fluorescence spectroscopy. Application in biochemistry.
4. Absorption methods. Colorimetry and spectrophotometry (UV/VIS and IC).
5. Atomic absorption spectrophotometric methods.
6. Charged bodies. Electric current. Introduction to electrochemistry.
7. Conductometry.
8. Potentiometry. Electric potential and electromotive force.
9. Direct potentiometric measurements.
10. Potentiometric titrations.
11. Electrogravimetric and coulometric methods - general principles.
12. Polarography and voltammetry - general principles.
13. Pulse techniques. Improving signal-to-noise ratio.

Exercises:

0 points (1 hour a week)

Labwork:

25 points (4 hours a week)

Syllabus:

• Weeks 1-4: Group exercises in optical methods (studying the hydrolysis of bromazepam, the binding of small molecules (warfarin or diazepam) to human serum albumin, ICP-OES analysis, determination of α-amino nitrogen, refractometric analysis of alcohols). 
• Week 5: A progress test on optical methods.
• Week 6: Group exercises in conductometry (determination of the temperature coefficient of sodium chloride, determination of the dependence of conductivity on concentration, determination of the dissociation constant of acetic acid).
• Week 7: A progress test on conductometry and individual exercises (acid-base or precipitation titration).
• Weeks 8-9: Group exercises in potentiometry (determination of fluoride content using an ion-selective electrode, determination of phosphoric acid in a real sample, determination of pK_a using a potentiometric method, determination of the stability constant of [Ag(NH₃)₂]⁺).
• Week 10: A progress test on potentiometry and individual exercises (precipitation or acid-base titration).
• Week 11: Group exercises in electrolysis (coulometric titrations of arsenic with generated bromine, coulometric titrations of arsenic with generated iodine).
• Week 12: A progress test on electrolysis and individual exercises (electrogravimetric analysis of copper or lead, coulometric analysis of HCl).
• Week 13: Group exercises in polarography and voltammetry (determination of half-wave potentials of Cu, Pb and Cd in two essential electrolytes, testing the reversibility of hexacyanoferrate(III)/ hexacyanoferrate (II) by cyclic voltammetry).
• Week 14: A progress test on polarography and voltammetry.

Semester papers:

5 points

Colloquia:

20 points

Written exam:

25 points

Oral exam:

25 points