Selected Chapters of Toxicological Biochemistry   (476B2)

Academic year:

2024/2025.

Attendance requirements:

There are no requirements.

ECTS:

10

Study level:

Study programs:

Teacher:

Milan R. Nikolić, Ph.D.
associate professor, Faculty of Chemistry, Studentski trg 12-16, Beograd

Assistants:

—

Hours of instruction:

Weekly: five hours of lectures + three hours of study research work

Goals:

Deepening knowledge of xenobiotics metabolism, their bioinactivation and bioactivation; free radical toxicology; interactions of reactive intermediates with biomolecules and implications of their toxicity; methods of identification, isolation and product determination of these interactions; the most significant classes of toxic substances and the biochemical basis of their effects on organ function.

Outcome:

The acquired knowledge will enable students to solve problems related to the testing of xenobiotic metabolism, to examine the toxic effects of foreign substances, to identify markers of toxic effects and to develop methods for their determination.

Teaching methods:

Lectures and seminar paper.

Extracurricular activities:

—

Coursebooks:

1. C. Ioannides (editor): Enzyme systems that metabolise drugs and other xenobiotics, Willey, 2002.
2. ş. B. Wallace (editor): Free radical toxicology, Taylor & Francis, 1997.
3. P. David Josephy: Molecular toxicology, Oxford University press, 1997.
4. R. J. M. Niesink: Toxicology - Principles and Applications, CRC, 1996.
5. Jurgen Augerer, Karl-Heinz Schaller (editors): Analyses of hazardous substances in biological materials, vol. 8, Wiley, 2003.
6. Selected original papers

Additional material:

—

Lectures:

10 points (5 hours a week)

Syllabus:

1. Enzyme systems involved in xenobiotic metabolism; Distribution in tissues, distribution in species, hormonal regulation, expression; Role in the activation and detoxification of foreign substances.
2. Isolation and characterization of xenobiotic-modified biomolecules; Identification of DNA adducts; Chemical mutagenesis, carcinogenesis and teratogenesis.
3. Free radical toxicology; Subcellular sites of their generation under the influence of xenobiotics; Lipid peroxidation; Protein and DNA modifications; Free radical defense; Examples of tissue damage by free radicals; Molecular and cellular aspects of free radical toxicity (changes in gene expression, changes in cellular signal transduction, chemical carcinogenesis).
4. Chemical substances - toxic agents - toxic effects; Toxic effects of gases, organic solvents, heavy metals, pesticides, food additives, drugs and radioactive materials.
5. Biochemical basis of the influence of toxic substances on the function of selected organs: hepatotoxicity, nephrotoxicity, hematotoxicity, neurotoxicity, genotoxicity, immunotoxicity.

Semester papers:

40 points

Oral exam:

50 points

Study research work:

0 points (3 hours a week)