Selected Chapters of Enzymology   (473B2)

Academic year:

2024/2025.

Attendance requirements:

There are no requirements.

ECTS:

10

Study level:

Study programs:

Teacher:

Dragana J. Stanić-Vučinić, Ph.D.
principal research fellow, Faculty of Chemistry, Studentski trg 12-16, Beograd

Assistants:

—

Hours of instruction:

Weekly: five hours of lectures + three hours of study research work

Goals:

The student deepens knowledge in the field of enzymatic kinetics, structure and activity of selected enzymes and deepens knowledge and skills related to solving complex kinetic problems. Part of the course Selected chapters of enzymology are created in connection with the chosen topic of the doctoral dissertation of the candidate.

Outcome:

The student deepened his/her knowledge in the field of enzymatic kinetics, structure and activity of selected enzymes.

Teaching methods:

Lectures, theoretical exercises, labwork, colloquia, semmester papers.

Extracurricular activities:

Student searches scientific literature, writes and defends seminar paper.

Coursebooks:

Main coursebooks:

• Alan Ferscht: Protein structure and mechanism.

Supplementary coursebooks:

• Review articles in the field of enzymology; review articles related to the selected doctoral dissertation topic.

Additional material:

—

Lectures:

10 points (5 hours a week)

Syllabus:

1. Mechanisms of enzyme reactions with one or more substrates.
2. Kinetics of enzymatic reactions.
3. Measurements of individual velocity constants.
4. Detection of intermediates in enzymatic reactions.
5. Mechanism of action of selected enzymes.
6. The technique of recombinant technology and its application in studying the mechanisms of enzymes catalyzed by reactions.
7. Protein engineering and testing of enzyme function and activity.

Semester papers:

30 points

Colloquia:

10 points

Oral exam:

50 points

Study research work:

0 points (3 hours a week)