Toxicological Biochemistry   (424B1)

Academic year:

2019/2020.

Attendance requirements:

201B1 + 202B1 + 062B1

ECTS:

7

Study level:

Study program:

Teacher:

Milan R. Nikolić, Ph.D.
associate professor, Faculty of Chemistry, Studentski trg 12-16, Beograd

Assistants:

—

Hours of instruction:

Weekly: three hours of lectures + four hours of labwork (3+0+4)

Goals:

The goal of this course is to help students acquire knowledge of the interactions of toxic (foreign) substances and the human body at the molecular level, biotransformations and bioactivation/bioinactivation of toxic substances, their interactions with bio(macro)molecules, and to help students master experimental skills necessary for working in toxicology laboratories.

Outcome:

Students understand: general concepts in toxicology; the process of biotransformation of foreign substances; the molecular aspects of the toxic effects of substances, the relationship between the structure and the activity of toxic substances; biochemical foundations of the manifestation of the toxicity of substances in target organs. Students have mastered the basic methods and techniques used in biochemical toxicology laboratories.

Teaching methods:

Lectures, theory exercises and experimental exercises, term papers.

Extracurricular activities:

—

Coursebooks:

Main coursebooks:

• Prof. dr źilan Jokanović: Toksikologija, Medicinski fakultet, Univerzitet u Nišu, 2010. (Beograd: Princip Press; ISBN 978-86-914075-0-6)
• Ljuba Mandić: Uputstva za vežbe iz toksikološke biohemije (interni materijal)
• P. David Josephy, Bengt Mannervik: Molecular Toxicology, Oxford University Press, 2006.
• Comprehensive Toxicology, 3^rd Edition (Editor-in-Chiefs: Charlene McQueen), Elsevier, 2017.

Supplementary coursebooks:

• Lectures' power-point presentations (Professor Dr. Ljuba Mandić)

Additional material:

—

Lectures:

10 points (3 hours a week)

Syllabus:

Teaching units:

1. The subject, historical development, multidisciplinary character and biochemical significance of toxicology as the "science of poisons";
2. Biochemical fate of toxic substances in the human body (intake, absorption, transport, distribution, deposition and elimination of toxicants);
3. Biotransformations of xenobiotics (phase I and phase II reactions, bio(in)activation and detoxification of toxicants) and the factors which influence these processes;
4. Biochemical mechanisms of the toxicity of xenobiotics (acute and chronic toxicity, mutagenicity, genotoxicity/carcinogenicity, teratogenicity of toxicants);
5. Toxic effects of xenobiotics/toxicants on target tissues and organs (blood, skin, liver, kidneys, lungs, nervous system).
   

Labwork:

20 points (4 hours a week)

Syllabus:

Topics:

1. Determination of the content of toxic substances and their metabolites.
2. Examination of the toxic effects of heavy metals.
3. Examination of the toxic effects of gases.
4. Examination of the toxic effects of organic solvents.
5. Examination of the toxic effects of pesticides.
6. Toxicity testing of xenobiotics.

Semester papers:

20 points

Oral exam:

50 points