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Course code:

Course name:
Toxicological Biochemistry

Academic year:


Attendance requirements:

201B1 + 202B1 + 062B1



Study level:

basic academic studies

Study programme:

Biochemistry: 4. year, winter semester, elective (E14B1) course


Milan R. NikoliŠ, Ph.D.
assistant professor, Faculty of Chemistry, Studentski trg 12-16, Beograd


Vesna B. JovanoviŠ, Ph.D.
scientific associate, Faculty of Chemistry, Studentski trg 12-16, Beograd

Hours of instruction:

Weekly: three hours of lectures + four hours of labwork (3+0+4)


This course will enable student to acquire basic knowledge about the interactions of toxic (foreign) substances and the human body at the molecular level, biotransformations and bioactivation/ bioinactivation of toxic substances, their interactions with bio(macro)molecules, and to master experimental skills for work in toxicological laboratories.


Understanding: basic toxicology concepts; biotransformation(s) of foreign substances; the molecular aspects of the substance(s) toxic effect(s), the relationship between the structure and the activity of toxic substance(s); biochemical foundation for the manifestation of substance(s) toxicity to target organs; mastering the basic methods and techniques used in toxicological biochemical laboratories.

Teaching methods:

Lectures, theoretical and experimental exercises, seminar paper.

Extracurricular activities:


Main coursebooks:

* Prof. dr ╝ilan Jokanović: Toksikologija, Medicinski fakultet, Univerzitet u Nišu, 2010. (Beograd: Princip Press; ISBN 978-86-914075-0-6)

* Ljuba Mandić: Uputstva za vežbe iz toksikološke biohemije (interni materijal)

* P. David Josephy, Bengt Mannervik: Molecular Toxicology, Oxford University Press, 2006.

* Comprehensive Toxicology, 3rd Edition (Editor-in-Chiefs: Charlene McQueen), Elsevier, 2017. 

Supplementary coursebooks:

Lectures' power-point presentations (Professor Dr. Ljuba Mandić)


Additional material:

Course activities and grading method


10 points (3 hours a week)


Teaching units:

1. Subject, historical development, multidisciplinary character and biochemical significance of toxicology as "poison science";

2. Biochemical fate of toxic substances in the human body (intake, absorption, transport, distribution, disposal and elimination of toxicants);

3. Biotransformation of xenobiotics (phase I and II reactions, bio(in)activation and detoxification of toxicants) and factors that influence these processes;

4. Biochemical mechanisms of xenobiotics toxicity (acute and chronic toxicity, mutagenicity, genotoxicity/ carcinogenicity, teratogenicity of toxicants);

5. Toxic effects of xenobiotics on target organs.


20 points (4 hours a week)


Topics of experimental exercises:

1. Determination of the content of toxic substances and their metabolites.

2. Examination of heavy metals toxic effects.

3. Examination of gases toxic effects.

4. Examination of toxic effects of organic solvents.

5. Examination of toxic effects of pesticides.

6. Xenobiotics toxicity testing.

Semester papers:

20 points

Oral exam:

50 points

Tue Dec 18 12:29:34 2018

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