[dr Natalija Dj. Polovic]

Faculty of Chemistry, Studentski trg 12-16, Beograd
Department of Biochemistry

Phone:   011-3336657, 011-3336721

Phone (local):   657, 721

Office:   420

Laboratory:   421

E-mail:   polovicn@chem.bg.ac.rs

Personal data

Born in Podgorica at 16. 4. 1979. Finished primary and secondary school in Bar.

Education and qualifications

1998-2002: Undergraduate studies - Chemistry / University of Belgrade - Faculty of Chemistry (Belgrade, Serbia)

2005: Master of science studies - Chemistry / University of Belgrade - Faculty of Chemistry (Belgrade, Serbia)

2008: Doctoral academic (PhD) studies - Chemistry / University of Belgrade - Faculty of Chemistry (Belgrade, Serbia)

2008-2009: Postdoctoral studies - Medicine / Karolinska Institutet (Stockholm, Sweden)

History of employment

2006-2010: Assistant / University of Belgrade - Faculty of Chemistry (Belgrade, Serbia)

2010-2016: Assistant professor / University of Belgrade - Faculty of Chemistry (Belgrade, Serbia)

2016-2021: Associate professor / University of Belgrade - Faculty of Chemistry (Belgrade, Serbia)

2021: Full professor / University of Belgrade - Faculty of Chemistry (Belgrade, Serbia)

Scientific interest

Identification, izolation, clonning, production, and characterization of proteins, protein biophysics. Amyloids. Protein denaturation; protein and RNA folding; protein and RNA-ligand interactions.

Awards and merits

2003: Kostic fund prize for extrodinary diploma work

2005: EAACI and GA2LEN Fellowship Award

2006: Best poster prize
XXV EAACI Congress, Vienna.Austria

2007: Best poster prize
XXVI EAACI Congress; Goteborg; Sweden

2008: IUPAC poster prize
46. Congress of the Serbian Chemical Society

2008: Best poster prize
XXVII EAACI Congress; Barcelona, Spain

2008: Djarmanovic fund and Royal Swedish Academy stipend

2009: Postdoc stipend. Ministry of Science and Technological Developement

2009: EAACI and GA2LEN Fellowship Award

2010: Abstract winner
XXVIII EAACI Congress, London, UK

Other activities

Member of Serbian Chemical Society from 2002.

Member of Serbian Biochemical Society from 2003.

Member of the Committee for Self-evaluation from November 9, 2017 till October 8, 2020.

Member of the Committee for Exams Recognition from January 18, 2018 till January 14, 2021.

Chairman of the Board of the Faculty of Chemistry from December 24, 2019.

Member of the Committee for Self-evaluation from October 8, 2020.

Member of the Committee for Exams Recognition from January 14, 2021.

Member of the Ethical Committee for Use of Human Biological Matherial in Research from July 12, 2021.

Functional amyloids for application in biotechnology, Serbia-Slovenia bilateral project 337-00-21/2020-09/27, 2021-22

Advanced Trends in Education and Research of Biochemistry, Biophysics and Biotechnology of Macromolecules, CEEPUS RS-1310, 2018-

Survey for antimicrobials effective against carbapenem resistant gram negative bacteria, CRP/SRB15-02 (collaboration with prof. dr Branko Jovcic). ICGEB; 2016-2018

Microbial cell surface determinants of virulence as targets for new therapeutics in Cystic Fibrosis, COST Action BM1003, 2010-2014

 

National project, 172049, 2010-2019;

National project, 142020, 2005-2010;

Design of a new chimeric protein for applications in specific immunotherapy of allergy to cat. EAACI, 2006

 

Development of novel allergy vaccines by molecular breeding. EAACI, 2009-2010.

Educational activities

Participation in research projects

Finished projects:

  • 2011: Allergens, antibodies, enzymes and small physiologically relevant molecules: design, structure, function and significance – 172049
    project type:   National Fundamental Research Projects
    project is funded by: Ministry of Science and Technological Development (Belgrade, Serbia)

Author identification numbers

Representative references

Knjige i revijalni radovi / Books and reviews:

  1. Natalija Polović, „Osnove biohemije“, 2021, Hemijski fakultet, Univerzitet u Beogradu, ISBN: 978-86-7220-106-2
  2. Ratko M Jankov, Marija Gavrović-Jankulović, Tanja Ćirković Veličković, Olgica Nedić, Zoran Vujčić, Natalija Polović, ²Praktikum iz Imunohemije² 2005, Hemijski fakultet, Univerzitet u Beogradu, ISBN 86-7220-022-5
  3. R. Jankov, N. Polović, T. Ćirković Veličković, “Praktikum – Hemija prirodnih proizvoda”, 2006, Hemijski fakultet, Univerzitet u Beogradu, ISBN: 86-7220-028-4
  4. Mucić, G., Rašković, B., Polović, N. Characterization of novel collagenolytic proteases (2017) Methods in Molecular Biology, 1626, pp. 71-78, https://doi.org/10.1007/978-1-4939-7111-4_7
  5. Jankovic, B.G., Polovic, N.D. The protein folding problem (2017) Biologica Serbica, 39, pp. 105-111, https://doi.org/10.5281/zenodo.827151
  6. Polovic, N., Velickovic, T.C. Novel formulations for oral allergen vaccination (2008) Recent Patents on Inflammation and Allergy Drug Discovery, 2 (3), pp. 215-221, https://doi.org/10.2174/187221308786241956

Izabrani radovi / Selected papers:

  1. Milošević, J., Janković, B., Prodanović, R., Polović, N. On the Protein Fibrillation Pathway: Oligomer Intermediates Detection Using ATR-FTIR Spectroscopy (2021) Molecules,26(4), 970; https://doi.org/10.3390/molecules26040970

     

    Vrhovac, L.S., Šelemetjev, S.A., Vatić, S., Mitrović, A., Milošević, J.R., Lolić, A.Đ., Beletić, A.D., Polović, N.Đ. Novel approach to the measurement of antithyroglobulin antibodies in human serum – application of the quartz crystal microbalance sensors (2021) Talanta, 223, art. no. 121588, https://doi.org/10.1016/j.talanta.2020.121588

     

     Vatić, S., Mirković, N., Milošević, J.R., Jovčić, B., Polović, N.Đ. Trypsin activity and freeze-thaw stability in the presence of ions and non-ionic surfactants (2021) Journal of Bioscience and Bioengineering, 131 (3), pp. 234-240, https://doi.org/10.1016/j.jbiosc.2020.10.010

     

     Vatić, S., Mirković, N., Milošević, J.R., Jovčić, B., Polović, N.Đ. Broad range of substrate specificities in papain and fig latex enzymes preparations improve enumeration of Listeria monocytogenes (2020) International Journal of Food Microbiology, 334, art. no. 108851, https://doi.org/10.1016/j.ijfoodmicro.2020.108851

     

     Milošević, J., Petrić, J., Jovčić, B., Janković, B., Polović, N. Exploring the potential of infrared spectroscopy in qualitative and quantitative monitoring of ovalbumin amyloid fibrillation (2020) Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy, 229, art. no. 117882, https://doi.org/10.1016/j.saa.2019.117882

     

    Milošević, J., Vrhovac, L., Đurković, F., Janković, B., Malkov, S., Lah, J., Polović, N.D. Isolation, identification, and stability of Ficin 1c isoform from fig latex (2020) New Journal of Chemistry, 44 (36), pp. 15716-15723 https://doi.org/10.1039/d0nj02938f

     

    Milošević, J., Janković, B., Prodanović, R., Polović, N. Comparative stability of ficin and papain in acidic conditions and the presence of ethanol (2019) Amino Acids, 51, pp. 829–838, https://doi.org/10.1007/s00726-019-02724-3

     

    Rašković, B., Vatić, S., Andelković, B., Blagojević, V., Polović, N. Optimizing storage conditions to prevent cold denaturation of trypsin for sequencing and to prolong its shelf life (2016) Biochemical Engineering Journal, 105, pp. 168-176, https://doi.org/10.1016/j.bej.2015.09.018

     

    Rašković, B., Popović, M., Ostojić, S., Andelković, B. Tešević, V., Polović, N. Fourier transform infrared spectroscopy provides an evidence of papain denaturation and aggregation during cold storage (2015) Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy, 150, pp. 238-246, https://doi.org/10.1016/j.saa.2015.05.061

     

    Rašković, B., Babić, N., Korać, J., Polović, N. Evidence of β-sheet structure induced kinetic stability of papain upon thermal and sodium dodecyl sulfate denaturation (2015) Journal of the Serbian Chemical Society, 80 (5), pp. 613-625, https://doi.org/10.2298/JSC140901007R

     

    Prokopovic, V., Popovic, M., Andjelkovic, U., Marsavelski, A., Raskovic, B., Gavrovic-Jankulovic, M., Polovic, N. Isolation, biochemical characterization and anti-bacterial activity of BPIFA2 protein (2014) Archives of Oral Biology, 59 (3), pp. 302-309, https://doi.org/10.1016/j.archoralbio.2013.12.005

     

    Raskovic, B., Bozovic, O., Prodanovic, R., Niketic, V., Polovic, N. Identification, purification and characterization of a novel collagenolytic serine protease from fig (Ficus carica var. Brown Turkey) latex (2014) Journal of Bioscience and Bioengineering, 118 (6), pp. 622-627, https://doi.org/10.1016/j.jbiosc.2014.05.020