Protein Therapeutics and Fundamentals of Bioinformatics   (433B2)

Academic year:

2024/2025.

Attendance requirements:

401B2

ECTS:

6

Study level:

Study program:

Teachers:

Marija Đ. Gavrović Jankulović, Ph.D.
full professor, Faculty of Chemistry, Studentski trg 12-16, Beograd

Milica M. Popović, Ph.D.
associate professor, Faculty of Chemistry, Studentski trg 12-16, Beograd

Assistants:

Isidora I. Protić Rosić, Ph.D.
teaching assistant, Faculty of Chemistry, Studentski trg 12-16, Beograd

Marija E. Tursunović
junior research assistant, Innovation center, Studentski trg 12-16, Beograd

Hours of instruction:

Weekly: two hours of lectures + three hours of labwork (2+0+3)

Goals:

The goal of this course is to help students acquire some new knowledge in the field of the design and production of protein therapeutics.

Outcome:

Students are able to design the structure of a protein therapeutic with the right properties and to predict its structure by using bioinformatics tools.

Teaching methods:

Lectures, experimental exercises, defending term papers.

Extracurricular activities:

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Coursebooks:

• Chris F. Van Der Walle (editor): Peptide and protein delivery, AP 2011.
• Ganapathy Subramanian (editor): Biopharmaceutical Production Technology, Wiley-VCH, 2012.
• Frank Breitling, Stefan Dubel: Recombinant antibodies, Wiley, 1999.
• Stefan H. E. Kaufmann: Novel vaccination strategies, Wiley-VCH, 2004.

Additional material:

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Lectures:

20 points (2 hours a week)

Syllabus:

The classification of protein therapeutics. Designing protein therapeutics by using bioinformatics tools. Predicting structural properties of a therapeutic protein. The production of protein therapeutics for diagnostic and therapeutic purposes: the selection of an expression system. Design and production of humanized monoclonal antibodies, antibody fragments. Design and production of fusion proteins, bifunctional fusion proteins, chimeric proteins. Cytokines and growth factors. Recombinant vaccines. Stability, folding, half-life. Delivery to target tissues. Protein therapeutics approved for human use. Good manufacturing practices for protein therapeutics: quality, safety, efficacy.

Labwork:

20 points (3 hours a week)

Syllabus:

Designing by using bioinformatics tools and the production of a recombinant therapeutic in the expression system. Isolation and characterization of a protein therapeutic by using modern biochemical and immunochemical methods. A term paper.

Semester papers:

10 points

Oral exam:

50 points