Course code:
Course name:

Academic year:


Attendance requirements:




Study level:

basic academic studies

Study program:

Biochemistry: 4. year, winter semester, compulsory course


Marija Đ. Gavrović Jankulović, Ph.D.
full professor, Faculty of Chemistry, Studentski trg 12-16, Beograd


Isidora I. Protić Rosić
assistant, Faculty of Chemistry, Studentski trg 12-16, Beograd

Hours of instruction:

Weekly: three hours of lectures + four hours of labwork (3+0+4)


The goal of this course is to help students acquire the basic knowledge in the field of immunology.


At the end of this course students will be familiar with the basic principles of the cellular and humoral immune response. Students will also master the techniques which are based on the antigen-antibody interaction through experimental work.

Teaching methods:

Lectures, lab work, colloquia, seminars.

Extracurricular activities:

Visit to the Institute of Virology, Vaccines and Sera Torlak, Belgrade; visit to the Institute for Application of Nuclear Energy (INEP), Belgrade.


  1. David Male, Jonathan Brostoff, David B. Roth, Ivan M. Roitt: Immunology, 8th Ed., Elsevier
  2. Laboratory Exercises in Immunochemistry, Faculty of Chemistry, Belgrade.

Additional material:

  Course activities and grading method


10 points (3 hours a week)


  • Introduction to Immunology.
  • Cells, tissues and organs of the immune system.
  • Mononuclear phagocytes in the immune defense.
  • The complement system.
  • The structure and function of immunoglobulins.
  • The basic principles of the immunochemical technique.
  • Human leukocyte antigens. Antigen presentation. T-cell receptor.
  • Maturation, activation and differentiation of B cells.
  • Maturation, activation and differentiation of T cells.
  • Cooperation between cells during an immune response.
  • The regulation of the immune response.
  • Types of hypersensitivity.
  • Autoimmunity.
  • Tumor immunology


15 points (4 hours a week)


  • Production of antiserum (immunization, bleeding) and testing the quality of the antiserum (immunodiffusion and immunoelectrophoresis techniques).
  • Preparation of an immunoaffinity matrix and isolation of antigens by immunoaffinity chromatography.
  • The immunoblot.
  • Enzyme-linked immunosorbent assay (ELISA).
  • Functional assays for the determination of the activation of the complement system.
  • Proliferation of T cells.
  • The application of fluorescence-activated cell sorting (FACS) in determination of surface markers of T cells.

Semester papers:

10 points


15 points

Oral exam:

50 points