Course code: 404B2 |
Course name: Methods in Biochemistry |
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Academic year: |
2024/2025. |
Attendance requirements: |
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ECTS: |
10 |
Study level: |
basic academic studies |
Study program: |
Biochemistry: 3. year, winter semester, compulsory course |
Teachers: |
Zoran M. Vujčić, Ph.D. Jelena Z. Radosavljević, Ph.D. |
Assistants: |
Aleksa D. Savić |
Hours of instruction: |
Weekly: three hours of lectures + seven hours of labwork (3+0+7) |
Goals: |
The main goal of this course is to help students master the basic techniques: the preparation of extracts, proteins and nucleic acids and the determination of their concentration. General chromatographic and FPLC techniques and electrophoresis. Independent processing of the data found in the literature. |
Outcome: |
Students prepared for independent problem solving in the isolation of proteins, nucleic acids and small molecules, their structural determination and characterization. Training students for their first steps in scientific research. |
Teaching methods: |
Lectures, experimental classes, tutorials. Students present their work using PowerPoint presentations. |
Extracurricular activities: |
— |
Coursebooks: |
Main coursebooks:
Supplementary coursebooks:
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Additional material: |
— |
Course activities and grading method |
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Lectures: |
0 points (3 hours a week) Syllabus:
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Labwork: |
10 points (7 hours a week) Syllabus: Students attend laboratory classes after they have successfully passed an entrance test. If a student does not pass the entrance test and has at least 51% of the total number of points, he has the right to take the entrance test one more time. Laboratory classes are organized in sets which last for 10 working days. 1-2. Preparation of crude extracts and determination of protein concentration. 3-4. Precipitation methods. 5-6. Matrix preparation, column packing. Determination of the molecular weight of a stained enzyme (HRPO). 7-10. Ion-exchange chromatography (IEC), hydrophobic interaction chromatography (HIC), immobilized metal affinity chromatography (IMAC), fast protein liquid chromatography (FPLC): Superdex, HIC, Mono Q. 10-12. Electrophoresis: native and isoelectric focusing, SDS PAGE, UT electrophoresis. 2D electrophoresis. 13-14. DNA. Isolation, quantification and analysis. Restriction digestion. Agarose electrophoresis. 14-15. RNA. Isolation, quantification and analysis. |
Written exam: |
30 points |
Oral exam: |
40 points |
Tutorial: |
20 points |