Academic year: |
2024/2025. |
Attendance requirements: |
There are no requirements. |
ECTS: |
9 |
Study level: |
graduate academic studies, integrated basic and graduate academic studies |
Study programs: |
Chemical Education: 5. year, winter semester, elective (E5AP2) course
Chemistry: 1. year, winter semester, elective (E51H2) course
Environmental Chemistry: 1. year, winter semester, elective (E52S2) course |
Teachers: |
Rada M. Baošić, Ph.D.
full professor, Faculty of Chemistry, Studentski trg 12-16, Beograd
Tatjana Ž. Verbić, Ph.D.
associate professor, Faculty of Chemistry, Studentski trg 12-16, Beograd
Aleksandar Đ. Lolić, Ph.D.
full professor, Faculty of Chemistry, Studentski trg 12-16, Beograd |
Assistant: |
Mladen M. Đurđević
junior research assistant, Institute of Chemistry, Technology and Metallurgy, Njegoševa 12, Beograd |
Hours of instruction: |
Weekly: four hours of lectures + two hours of exercises + three hours of labwork (4+2+3) |
Goals: |
Students will gain and/or complete their knowledge in various instrumental techniques used in analytical, environmental, pharmaceutical and medicinal chemistry. |
Outcome: |
Students will be qualified for independent research, optimization and sensitivity improvement of various instrumental methods for the analysis of complex samples. |
Teaching methods: |
Lectures, theoretical exercises, lab work, semester paper. |
Extracurricular activities: |
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Coursebooks: |
Main coursebooks:
- J. A. C. Broekaert: Analytic Atomic Spectroscopy with Flames and Plasmas, John Wiley & Sons, Inc., Hoboken, New Jersey, USA.
- J. Wang: Analytical Electrochemistry, John Wiley & Sons, Inc., Hoboken, New Jersey, USA.
- A. J. Bard, L. R. Faulkner: Electrochemical methods, fundamentals and application, John Wiley & Sons, Inc., Hoboken, New Jersey, USA.
- A. Lolić: Protočna injekciona analiza - principi i primena, Univerzitet u Beogradu - Hemijski fakultet, Beograd, 2023. (in Serbian)
- F. Settle: Handbook of instrumental techniques for analytical chemistry, Prentice Hall PTR, Upper Saddle River, NJ 07458
- J. R. Lakowicz: Principles of Fluorescence Spectroscopy, Springer Science Business Media, New York, USA.
- Z. Mandić (editor): Physico-Chemical Methods in Drug Discovery and Development, IAPC Publishing, Zagreb, Croatia, 2012.
Supplementary coursebooks:
- T. Verbić, M. Pešić, N. Obradović, I. Cvijetić: Praktikum sa zbirkom zadataka iz instrumentalne analitičke hemije - optičke i elektroanalitičke metode, Univerzitet u Beogradu - Hemijski fakultet, 2018.
Lectures and manuals for lab work will be provided during the course through Faculty network. |
Additional material: |
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Course activities and grading method |
Lectures: |
5 points (4 hours a week)
Syllabus:
- Modern optical methods, introduction.
- Atomic absorption and emission spectroscopy.
- Flow-injection analysis.
- Spectroelectrochemistry.
- Cyclic and stripping voltammetry.
- Pulse voltammetric techniques.
- Electrochemical sensors and electrode modifications.
- Electrochemical methods of separation.
- Hybrid techniques.
- Application of instrumental methods in environmental chemistry.
- Potentiometry, potentiometric titrations and potentiometric biosensors.
- Fluorescence spectroscopy (FS) - principles and application.
- Capillary electrophoresis (CE) - principles and application.
- Detection methods in liquid chromatography.
- Equilibria in analytical chemistry - methods for determination of dissociation and stability constants, lipophilicity and solubility.
- Experimental techniques for measurements of plasma proteins - small molecules binding.
- Isothermal titration calorimetry (ITC) - principles and application.
- Surface plasmon resonance (SPR) - principles and application.
- źolecularly imprinted polymers (MIPs).
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Exercises: |
0 points (2 hours a week)
Syllabus:
Theoretical exercises are combined with lab work. During the long-lasting experiments attention will be paid to particular case studies related to the on-going thematic unit addressed during lectures and laboratory exercises. |
Labwork: |
20 points (3 hours a week)
Syllabus:
- Matrix effect on metal determination by flame atomic absorption spectrometry.
- Determination of heavy metals by electrothermal atomic absorption spectrometry.
- Determination of ascorbic acid by cyclic voltammetry in fresh fruits and vitamin C tablets.
- Determination of ascorbic acid by flow injection analysis.
- Determination of metals by flow injection analysis on modified carbon electrode.
- Determination of diazepam on modified screen-printed electrode.
- Ion-selective elecrodes: application of nitrate-selective and iodide-selective electrodes in real samples analysis. Sensors for oxygen and carbon dioxide.
- Fluorescence spectroscopy: examination of small biologically active molecule – HSA interactions.
- Chromatographic examination of propranolol binding to AGP. Separation of propranolol enantiomers on chiral HPLC column.
- Fluorescence polarization: determination of progesteron in milk by immunoassay.
- Molecularly imprinted polymers: conductometric study of monomer–template interactions in prepolymerization mixture. Preparation of molecularly imprinted (MIP) and non-imprinted polymer (NIP).
- Molecularly imprinted polymers: study of template binding to MIP and NIP by HPLC. NMR spectroscopic study of the template–polymer interactions.
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Semester papers: |
20 points |
Oral exam: |
55 points |