Course code:
256H2		 Course name:
Modern Structural Methods

Academic year:

2024/2025.

Attendance requirements:

There are no requirements.

ECTS:

9

Study level:

graduate academic studies, integrated basic and graduate academic studies

Study programs:

Chemical Education: 5. year, winter semester, elective (E5AP2) course

Chemistry: 1. year, winter semester, elective (E53H2) course

Teachers:

Gordana B. Krstiæ, Ph.D.
assistant professor, Faculty of Chemistry, Studentski trg 12-16, Beograd

Ivana V. Sofreniæ, Ph.D.
assistant professor, Faculty of Chemistry, Studentski trg 12-16, Beograd

Assistants:

—

Hours of instruction:

Weekly: four hours of lectures + two hours of exercises + three hours of labwork (4+2+3)

Goals:

Students should get acquainted with the following areas of instrumental methods:

  1. Multipulse 1D, 2D and 3D NMR methods (H,H-COSY, TOCSY, H,H-NOESY, ROESY, HETCOR, INADEQATE, and selected 3D experiments), semi-solid state NMR (HR-MAS NMR) as well as their application.
  2. Basic principles of tandem mass spectrometry (MS/MS) and its application in analytics.
  3. Application of MS to determine the structure of thermolabile molecules such as, polypeptides, sugars, etc.

Outcome:

Students will be trained for solving the structures of complex organic molecules using multi-pulsed NMR experiments. They will acquire the necessary knowledge and skills to identify and quantify them using modern mass spectrometry methods.

Teaching methods:

Lectures, theoretical exercises, labwork, homework and colloquia.

Extracurricular activities:

Guest lectures.

Coursebooks:
  1. Slobodan Milosavljević: Strukturne instrumentalne metode
  2. Vele Tešević: Osnove masene spektrometrije organskih jedinjenja
  3. Ernö Pretsch, Philippe Bühlmann and Martin Badertscher: Structure determination of organic compounds, Tables of Spectral Data
  4. L. D. Field, S. Sternhell, J. R. Kalman: Organic structures from spectra, fourth edition

Additional material:

—

  Course activities and grading method

Lectures:

0 points (4 hours a week)

Syllabus:

  1. Multipulse 1D and 2D NMR methods.
  2. H,H-COSY, TOCSY.
  3. Pulse sequences for suppression of signal in the spectrum (watergate, CPMG).
  4. H,H-NOESY, ROESY.
  5. HETCOR.
  6. INADEQATE.
  7. Protein-ligand screening STD (saturation transfer difference) NMR.
  8. Selected 3D NMR experiments.
  9. Insoluble Substance Analysis - Semi-solid NMR, HR-MAS assay.
  10. Soft ionization methods in MS.
  11. Tandem mass spectrometry (quadrupole, ion trap, ion cyclotron, orbitrap, etc.).
  12. MS/MS experiments of qualitative and quantitative targeted analysis (MRM, SRM, daughter scan and neutral loss).
  13. Electronic Paramagnetic Resonance (EPR) - Basic Principles and Application.

Exercises:

0 points (2 hours a week)

Labwork:

10 points (3 hours a week)

Syllabus:

  1. Structure elucidation of organic compounds based on H,H-COSY and TOCSY spectra.
  2. Structure elucidation of organic compounds based on HETCOR spectra.
  3. Structure elucidation of organic compounds based on INADEQATE spectra.
  4. Structure elucidation s of organic compounds based on H, H-NOESY and ROESY spectra.
  5. Introduction to the existing equipment of the Faculty of Chemistry (GC, LC, MS, GC/MS, LC/MS); sample preparation, spectra capture and interpretation. NMR, 2D NMR).
  6. Antioxidative activity (DPPH radical assay) of various sample types.
  7. HPTLC - Bioautography assay.
  8. Methods for fraud detection in food supplements.
  9. Secondary structure determination of proteins using FTIR spectroscopy.
  10. Quantitative NMR analysis (qNMR).
  11. STD NMR spectroscopy: Protein-ligands screening.
  12. NMR in quality control of Heparin Sodium.

Colloquia:

30 points

Written exam:

30 points

Oral exam:

30 points