Academic year: |
2024/2025. |
Attendance requirements: |
There are no requirements. |
ECTS: |
4 |
Study level: |
graduate academic studies |
Study program: |
Biochemistry: 1. year, winter semester, elective (E52B2) course |
Teacher: |
Radivoje M. Prodanoviæ, Ph.D.
full professor, Faculty of Chemistry, Studentski trg 12-16, Beograd |
Assistant: |
Ana V. Budimiroviæ |
Hours of instruction: |
Weekly: two hours of lectures + two hours of labwork (2+0+2) |
Goals: |
Students should get knowledge about state of the art of protein engineering and design. |
Outcome: |
Know how of theoretical and experimental methods for protein engineering and design. |
Teaching methods: |
Lectures, labwork, semmester papers. |
Extracurricular activities: |
— |
Coursebooks: |
- À. ¿àÞÔÐÝÞÒØû: ¿àÞâÕØÝáÚØ ØÝÖÕúÕàØÝÓ, ÃÝØÒÕà×ØâÕâ ã ±ÕÞÓàÐÔã - ÅÕÜØøáÚØ äÐÚãÛâÕâ, 2019.
- Sheldon J. Park, Jennifer R. Cochran: Protein Engineering and Design, CRC Press 2010.
- S. Brakmann, K. Johnsson: Directed Molecular Evolution of Proteins, WILEY-VCH 2002.
- Selected review papers
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Additional material: |
— |
Course activities and grading method |
Lectures: |
10 points (2 hours a week)
Syllabus:
- Theories of protein evolution and in silico protein design.
- In vitro protein evolution - principles and troubleshooting.
- Surface display of proteins in Protein engineering.
- In vitro translation of proteins in Protein engineering.
- Gene library preparation - gene duplication, circular permutations, gene fusion, exon shuffling, mutations, consensus mutagenesis.
- High throughput screening methods in Protein engineering - principles and troubleshooting.
- Bioinformatics in Protein engineering.
|
Labwork: |
20 points (2 hours a week)
Syllabus:
- Preparation of gene libraries by saturation mutagenesis, recombination and error prone PCR.
- Transformation of yeast S. cerevisie and expression of gene library on the surface of yeast cells.
- High throughput screening of gene libraries in microtiter plates.
- High throughput screening of gene libraries using in vitro compartmentalization and flow cytometry.
|
Semester papers: |
20 points |
Oral exam: |
50 points |