Course code:
452B2		 Course name:
Molecular Biotechnology

Academic year:

2024/2025.

Attendance requirements:

There are no requirements.

ECTS:

8

Study level:

graduate academic studies

Study program:

Biochemistry: 1. year, winter semester, compulsory course

Teacher:

Tanja D. Ćirković Veličković, Ph.D.
full professor, Faculty of Chemistry, Studentski trg 12-16, Beograd

Assistants:

Mirjana Ž. Radomirović
research assistant, Faculty of Chemistry, Studentski trg 12-16, Beograd

Višnja D. JovanovićFaculty of Chemistry, Studentski trg 12-16, Beograd

Marija D. Stefanović
junior research assistant, Institute of Chemistry, Technology and Metallurgy, Njegoševa 12, Beograd

Hours of instruction:

Weekly: three hours of lectures + five hours of labwork (3+0+5)

  Course activities and grading method

Lectures:

10 points (3 hours a week)

Syllabus:

  1. An introduction to molecular biotechnology. Principles and applications of recombinant DNA Molecular biotechnology biological systems (procariotic and eukariotic) Eukariotic cells in culture DNA, RNA and protein synthesis.
  2. An introduction to molecular biotechnology. An overview of standard techniques applied in molecular biotechnology - including chromatography and electrophoresis, cloning techniques, gene expression systems, immunological methods, labeling of proteins and in situ-techniques, microscopy and laser systems.
  3. Cloning strategies Restriction endonucleases Plasmid cloning vectors Creating and screening a library Transformation of procariots cDNA libraries.
  4. Chemical synthesis and sequencing of DNA Amplification of DNA Sequencing of DNA.
  5. Gene manipulation strategies (protein engineering) Rational design of proteins Site-directed mutagenesis Dirigated evolution of proteins.
  6. Heterologous expression of proteins in procariotic systems.
  7. Manipulation of gens in procariots and optimization of gene expression in procariots.
  8. Heterologous expression of proteins in eucariotic systems.
  9. Saccharomyces cerevisiae expression system Other yeast expression systems Insect cells expression systems.
  10. Molecular biotechnology of microbial systems.
  11. Molecular diagnostics Microbial production of therapeutic agents.
  12. Vaccines.
  13. Synthesis of commercial products by recombinant microorganisms Restriction endonucleases Antibiotics Biopolymers Other examples.
  14. Microbial insecticides.
  15. Genetic engineering of plants: methodology and applications.
  16. Animal biotechnology.
  17. Transgenic animals.
  18. Regulating and patenting molecular biotechnology Ethical concerns in molecular biotechnology Patenting molecular biotechnology Legal, social and ethical aspects of biotechnology.

Labwork:

10 points (5 hours a week)

Colloquia:

20 points

Written exam:

60 points